Transformed plant-culture by E.coli heat labile toxin B sub-unit gene, vaccine comprising thereof

摘要

PURPOSE: A plant culture which is transformed with an E.coli enterotoxin B subunit gene is provided to enable oral administration in a digestive organ. CONSTITUTION: An expression vector contains E.coli enteritoxin B subunit gene between rice amylase 1A promoter and 3'-untranslated region. A hygromycin phosphotransferase gene is coated as a selection marker. The subunit gene is denoted by sequence number 1. A vaccine composition for preventing diarrhea contains a plant culture transformed by a gene encoding the B subunit or plant culture extract. A food additive for preventing diarrhea contains the plant culture. The vaccine composition is used for oral administration.