MANGENESE PEROXIDASE CODING GENE SEPARATED FROM WHITE ROT FUNGI PHANEROCHAETE CHRYSOSPORIUM BKM-F-1767 AND THEIR EXPRESSION METHOD

摘要

PURPOSE: A gene encoding manganese peroxidase derived from Phanerochaete chrysosporium BKM-F-1767 and an expression method thereof are provided to produce bioethanol. CONSTITUTION: A gene encoding manganese peroxidase(MnP) has a base sequence of sequence number 1, derived from Phanerochaete chrysosporium BKM-F-1767 and an amino acid of sequence number 2. An expression method of a gene encoding MnP comprises: a step of inserting a gene encoding MnP into a pPICZαA expression vector and preparing a recombinant expression vector; a step of transducing the recombinant expression vector into Pichia pastoris GS115 and transforming with an alcoholoxide I(AOX 1) promoter in the vector for extracellular expression. An enzyme agent for decomposing ligneous biomass contains expressed MnP as an active ingredient.