METHOD FOR ISOLATING INTRACELLULAR ANTIBODIES ABLE TO NEUTRALIZE PROTEIN INTERACTIONS

摘要

A method is described for isolating neutralizing intracellular antibodies of an interaction between a protein ligand x and a protein ligand y inside a cell. A method to identify a protein ligand x able to bind to a known y ligand using intracellular antibodies able to disrupt the interaction between x and y is also described. A method for the isolation of a set of antibody fragments against a significant proportion of the protein-protein interactions of a given cell (interactome) or against the protein interactions that constitute an intracellular pathway or network is also described.