| 摘要 |
PURPOSE: A primer pair and probe for real-time PCR primer for diagnosing avellino cornea dystrophy are provided to quickly and accurately diagnose mutation of exon 4 of BIGH3 gene. CONSTITUTION: A PCR primer pair for real-time PCR for avellino corneal dystrophy diagnosis is denoted by sequence numbers 3 and 4. A kit for diagnosing Avellino cornea dystrophy contains the primer pair of sequence numbers 3-4 and PCR probe of sequence number 25 or 26. The PCR probe is labeled with VIC or FAM. |
