METHOD FOR DETERMINING THE GENOTYPE OF MULTIPLE SEQUENCES

摘要

Method for determining the genotype of multiple sequences. A method is disclosed for the determination of the genotype of multiple sequences of interest, in particular single nucleotide polymorphisms (SNPs). The method uses a single amplification reaction for the amplification of the target sequences that comprise multiple sequences of interest, in particular SNP sites, and a single genotyping reaction for the sequencing of multiple sequences of interest, in particular SNP sites, in a simultaneous manner. The method reduces the number of reactions needed to sequence small regions of interest in longer lengths of DNA of little interest. The method also reduces the cost of the tests that would otherwise require a high number of sequencing reactions, reduces the amount of starting template DNA required for sequencing multiple regions of interest, and yields a product that is technically less complex.