发明名称 |
METHOD FOR GENOME COMPLEXITY REDUCTION AND POLYMORPHISM DETECTION |
摘要 |
The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.
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申请公布号 |
US2013035238(A1) |
申请公布日期 |
2013.02.07 |
申请号 |
US201213535190 |
申请日期 |
2012.06.27 |
申请人 |
KIRST MATIAS;RIBEIRO DE RESENDE, JR. MARCIO FERNANDO;NEVES LEANDRO GOMIDE;DERVINIS CHRISTOPHER;BALMANT KELLY MAYRINK |
发明人 |
KIRST MATIAS;RIBEIRO DE RESENDE, JR. MARCIO FERNANDO;NEVES LEANDRO GOMIDE;DERVINIS CHRISTOPHER;BALMANT KELLY MAYRINK |
分类号 |
C40B50/06;C07H21/04;C12N9/16;C40B20/00 |
主分类号 |
C40B50/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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