IMPROVED MICROFLUIDIC DEVICE AND METHOD AND SYSTEM FOR ITS USE

摘要

A method, device, and system is disclosed to detect whether one or more analytes of interest are present on a cell or cell organelle surface. The device includes a cartridge that includes a channel having a bottom surface that includes at least one immobilized patch of reagents with a binding affinity for one of the analytes of interest. The method introduces a fluid containing at least one cell or cell organelle into the channel, measures the velocity of at least one cell or cell organelle individually over each of the immobilized patches at a plurality of defined time intervals or distances for each patch, discards some of the largest velocity measurements, and compares the mean value of the remaining velocity measurements to a control value to determine whether the analyte of interest is present on the surface of the cell or cell organelle.