发明名称 |
FLUORESCENCE-BASED APPROACH TO MONITOR RELEASE FACTOR-CATALYZED TERMINATION OF PROTEIN SYNTHESIS |
摘要 |
Provided are probes comprising a class 1 release factor conjugated to a fluorescent label and methods of making the probes. Also provided are methods for detecting conformational changes in ribosomes and associated molecules, such as class 1 release factors. In addition, methods of identifying a compound for reducing nonsense-mediated decay of mRNA and/or for inhibiting termination of protein synthesis at a premature stop codon, are described. Methods of assaying RF3 activity are also included.
|
申请公布号 |
US2013034914(A1) |
申请公布日期 |
2013.02.07 |
申请号 |
US201213407438 |
申请日期 |
2012.02.28 |
申请人 |
GONZALEZ, JR. RUBEN L.;STERNBERG SAMUEL H.;PULUKKUNAT DILEEP K. |
发明人 |
GONZALEZ, JR. RUBEN L.;STERNBERG SAMUEL H.;PULUKKUNAT DILEEP K. |
分类号 |
C07K14/00;C07K1/113;G01N21/64 |
主分类号 |
C07K14/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|