| 摘要 |
The present invention describes a method, based on a semi-nested polymerase chain reaction (PCR), for the detection and identification of ten clinically relevant Candida species. The method involves a first PCR amplification of the internal transcribed spacer region ITS2 of fungal ribosomal RNA, followed by a second PCR reaction with a species-specific primer. Custom-designed primers are provided.
|
