发明名称 |
A process for purifying vitamin K dependent proteins such as coagulation factor IX |
摘要 |
A process of manufacturing a prion-free Vitamin K dependent Protein in a purification sequence employing chromatography characterized in that at least one chromatography step is performed using a multimodal resin providing a fraction containing Vitamin K dependent Protein in an aqueous solution; contacting the fraction containing the Vitamin K dependent Protein with a multimodal resin at a pH between 6-9; optionally washing the multimodal resin having the Vitamin K dependent Protein adsorbed with an aqueous washing buffer to wash away contaminants and retain the Vitamin K dependent Protein, before the Vitamin K dependent Protein is eluted; the Vitamin K dependent Protein is eluting from the multimodal resin at a pH between 6 to 9 in a buffer comprising arginine; and optionally collecting Vitamin K dependent Protein containing fractions in purified or enriched form. |
申请公布号 |
EP2553095(A1) |
申请公布日期 |
2013.02.06 |
申请号 |
EP20110713216 |
申请日期 |
2011.03.30 |
申请人 |
OCTAPHARMA AG |
发明人 |
GILLJAM, GUSTAV;WINGE, STEFAN |
分类号 |
C12N9/64 |
主分类号 |
C12N9/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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