| 摘要 |
An object is to identify endoglucanase and ²-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or ²-glucosidases, from Acremonium cellulolyticus , and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and ²-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus , and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and ²-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and ²-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed. |
