发明名称 |
TEMPERATURE-RESPONSIVE CELL CULTURE SUBSTRATE ON WHICH A STRAIGHT-CHAIN TEMPERATURE-RESPONSIVE POLYMER IS IMMOBILIZED, AND MANUFACTURING METHOD THEREFOR |
摘要 |
<p>Provided is a temperature-responsive cell culture substrate. A non-crosslinked temperature-responsive polymer having a molecular weight between 10,000 and 150,000 is immobilized on the substrate surface with a density of 0.02 to 0.3 molecular chain per square nanometer. Using the provided temperature-responsive cell culture substrate, cells obtained from various tissues can be efficiently cultured. This culturing method makes it possible to efficiently peel off a cell sheet by just changing the temperature, without causing damage.</p> |
申请公布号 |
EP2471899(A4) |
申请公布日期 |
2013.02.06 |
申请号 |
EP20100812012 |
申请日期 |
2010.08.27 |
申请人 |
TOKYO WOMEN'S MEDICAL UNIVERSITY |
发明人 |
TAKAHASHI, HIRONOBU;NAKAYAMA, MASAMICHI;OKANO, TERUO |
分类号 |
C12M3/00;C12N5/071 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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