| 摘要 |
<p>PURPOSE: A method for proliferating placenta-derived stem cells is provided to suppress stem cell differentiation and to maintain stemness. CONSTITUTION: A method for proliferating placenta-derived stem cells comprises a step of sub-culturing the placenta-derived stem cells under a hypoxic condition. The stem cells are amniotic mesenchymal stem cells which are derived from amnion of human placenta or amniotic epithelial cells of human placenta. The sub-culture is performed in an alpha-MEM medium containing 10% FBS, 1% of penicillin-streptomycin, 1ug/ml of heparin, and 25 ng/ml of FGF-4(Fibroblast Growth Factor-4).</p> |
