METHOD AND KIT FOR DETECTING HUMAN PAPILLOMA VIRUS

摘要

The present invention relates to a method and kit for detecting the type of a human papilloma virus. The method comprises: (1) a step of a first PCR amplification of an L1 gene from the sample to be analyzed, using a first primer which is specific to the L1 gene of human papilloma virus (HPV) and a second primer which is both specific to the L1 gene of human papilloma virus (HPV) and marked with a marker recognized by an exonuclease; (2) a step of adding the exonuclease recognizing said marker to the amplified L1 gene product, and then decomposing a nucleic acid strand extending from the second primer marked with said marker so as to obtain an L1 gene product having a single strand extending from the first primer; (3) a step of a second PCR amplification of the L1 gene into a single strand using, as a template, the obtained single strand extending from the first primer and using the second primer which is not marked with the marker recognized by the exonuclease; (4) a step of a hybridization reaction of the nucleic acid having the amplified single strand with a probe for detecting a human papilloma virus type; and (5) a step of measuring a signal from the hybridized product in order to check the type of a human papilloma virus. Using the probe for detecting a human papilloma virus type and the method and kit for detecting a human papilloma virus type provided by the present invention, types of human papilloma viruses can be detected accurately and with high sensitivity. According to the present invention, a highly accurate and reliable, simple, and highly sensitive detection method enables an early diagnosis of a trace amount of human papilloma viruses existing prior to the proliferation of same.