CONDITIONALLY IMMORTALIZED LONG-TERM STEM CELL, AND METHOD FOR MAKING AND USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a method for making, maintaining and manipulating stable cell lines that can give rise to all cell lineages that would normally arise when placed under appropriate conditions.

SOLUTION: This method for producing conditionally immortalized adult stem cells includes steps of: (a) obtaining a proliferated population of adult stem cells; (b) transfecting the stem cells with a nucleic acid molecule comprising a protooncogene or biologically active fragment or homologue thereof that promotes cell survival and proliferation, wherein the protooncogene is inducible; (c) transfecting the stem cells with a nucleic acid molecule encoding a protein that inhibits apoptosis of the cell; and (d) proliferating the transfected cells in the presence of a combination of stem cell growth factors under the condition that the protooncogene is active, to produce conditionally immortalized adult stem cells.

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