发明名称 |
DETECTING METHOD OF SEVERAL FOOD-POISONING BACTERIA USING PNA CHIP |
摘要 |
<p>PURPOSE: A method for detecting poisoning bacteria using a PNA microarray chip, a PNA and PNA microarray chip are provided to detect six kinds of food-poisoning bacteria with high sensitivity and specificity and to easily and systemically control the food-poisoning bacteria. CONSTITUTION: A PNA(peptide nucleic acid) probe for detecting food poisoning bacteria has a base sequence selected from sequence numbers 1-7. The PNA probe with a base sequence of sequence number 1 specifically binds to Bacillus cereus. The PNA probe with a base sequence of sequence number 2 specifically binds to Listeria monocytogenes. The PNA probe with a base sequence of sequence number 3 specifically binds to Staphylococcus aureus. The PNA probe with a base sequence of sequence number 4 specifically binds to Yersinia enterocolitica. The PNA probe with a base sequence of sequence number 5 specifically binds to Salmonella enteric. A method for detecting poisoning in a sample comprises a step of hybridizing the PROBE with the sample; and a step of detecting a signal generated by hybridization.</p> |
申请公布号 |
KR20130010974(A) |
申请公布日期 |
2013.01.30 |
申请号 |
KR20110071815 |
申请日期 |
2011.07.20 |
申请人 |
REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION) |
发明人 |
HEU, SUNG GI;YUN, JONG CHUL;JUNG, KYU SUK;ROH, EUN JUNG;PARK, JOO WON |
分类号 |
C12N15/11;C12Q1/04;C12Q1/68 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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