| 摘要 |
<p>The present invention provides a method for selectively recovering fetal cells from a maternal blood sample. The method comprises the following steps. Cells of the sample are combined with a first and a second antibody labeled with different fluorochromes for a period of time sufficient for antibody binding to produce labeled cells. The antibodies are specific for two different antigens expressed by two material HLA alleles. In a preferred embodiment, the alleles are of an HLA locus for which the woman is heterozygous. Cells having two different fluorescent labels are separated from cells having either a single fluorescent label or unlabeled cells using fluorescence-activated cell sorting. The separated single-labeled and unlabeled cells are recovered. The separated fetal cells can be used in a variety of procedures including DNA amplification methods and karyotyping.</p> |
