发明名称 |
Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell |
摘要 |
In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
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申请公布号 |
US8361982(B2) |
申请公布日期 |
2013.01.29 |
申请号 |
US20100769157 |
申请日期 |
2010.04.28 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;LOIS-CABALLE CARLOS;BALTIMORE DAVID;QIN XIAO-FENG;CHEN IRVIN S. Y.;AN DONG SUNG |
发明人 |
LOIS-CABALLE CARLOS;BALTIMORE DAVID;QIN XIAO-FENG;CHEN IRVIN S. Y.;AN DONG SUNG |
分类号 |
A01N43/04;C12N15/09;A61K31/7088;A61K48/00;A61P31/18;C07H21/04;C12N15/00;C12N15/113;C12N15/867;C12P19/34;C12Q1/68 |
主分类号 |
A01N43/04 |
代理机构 |
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地址 |
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