发明名称 |
Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme |
摘要 |
Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.
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申请公布号 |
US8361769(B1) |
申请公布日期 |
2013.01.29 |
申请号 |
US20090619033 |
申请日期 |
2009.11.16 |
申请人 |
U.S. DEPARTMENT OF ENERGY;KOCH DANIEL J.;ARNOLD FRANCES H. |
发明人 |
KOCH DANIEL J.;ARNOLD FRANCES H. |
分类号 |
C12N9/02;C07C9/02;C12P21/02 |
主分类号 |
C12N9/02 |
代理机构 |
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地址 |
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