发明名称 |
Process for preparing variant of Erysipelothrix rhusiopathiae surface protective antigen in E. coli |
摘要 |
A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (&Dgr;SpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or &Dgr;SpaA protein provides a variant of SpaA or &Dgr;SpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or &Dgr;SpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.
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申请公布号 |
US8361749(B2) |
申请公布日期 |
2013.01.29 |
申请号 |
US20100949606 |
申请日期 |
2010.11.18 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE;USHIJIMA TOSHIHIRO;SAKAGUCHI MASASHI;TOKUNAGA EIJI |
发明人 |
USHIJIMA TOSHIHIRO;SAKAGUCHI MASASHI;TOKUNAGA EIJI |
分类号 |
A61K39/00;A61K38/00;A61K38/16;A61K39/02;A61K39/38;A61P31/00;C07K14/195;C12N15/00;C12N15/09;C12P21/02;C12P21/06 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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