CELL CULTURE APPARATUS, APPARATUS FOR LONG-TERM OBSERVATION OF CELL CULTURE, METHOD FOR LONG-TERM CELL CULTURE, AND METHOD FOR LONG-TERM OBSERVATION OF CELL CULTURE

摘要

Provided are a cell culture device, a device for long-term monitoring of cell culture, a method for long-term cell culture, and a method for long-term monitoring of cell culture, with which it is possible to culture and monitor cells continuously under uniform environmental conditions and to trace a specific cell history (genealogy) without the changes in physical state that accompany aging of culture cells. The cell culture device comprises a cell culture substrate, a semipermeable membrane, and culture liquid feed means, wherein the cell culture substrate has at the surface a thin culture groove for carrier culture of cells and a thick discharge groove for discharge of cells that have been carrier cultured in the culture groove; both ends of the culture groove are connected to the discharge groove; the discharge groove is thicker and deeper than the culture groove; and the semipermeable membrane covers the culture groove and the discharge groove of the cell culture substrate. The culture liquid feed means are capable of continuously feeding culture liquid to the cell culture substrate covered by the semipermeable membrane.