发明名称 |
Recycling of enzymes from bioreactors |
摘要 |
A method of conducting an enzymatic process in which the enzymes are recovered and reused in at least a second iteration of the process, wherein each iteration of the process comprises the steps of: (a) providing a heterogeneous substrate solution, in which the substrate is fully soluble, partially soluble or insoluble; (b) adding an enzyme or mixture of enzymes to the heterogeneous substrate solution; and (c) allowing an enzymatic reaction of the substrate to proceed; wherein, after completion of step (c) for each iteration, the enzyme is recovered from the mixture resulting from step (c) according to the following steps: (d) conducting a non-packed-bed adsorption process comprising contacting the reaction mixture with an adsorbent that adsorbs the enzyme in order to separate the enzyme from the reaction mixture resulting from step (c); (e) optionally washing unbound material from the adsorbent; and (f) desorbing the enzyme from the adsorbent; and further wherein the desorbed enzyme obtained in step (f) is used in step (b) of at least one subsequent iteration of the process. |
申请公布号 |
AU2011268877(A1) |
申请公布日期 |
2013.01.24 |
申请号 |
AU20110268877 |
申请日期 |
2011.06.24 |
申请人 |
UPFRONT CHROMATOGRAPHY A/S |
发明人 |
LIHME, ALLAN OTTO FOG;STANLEY, CHRISTOPHER JOHN |
分类号 |
C12P7/02;C12P7/06;C12P7/08;C12P7/10;C12P7/16;C12P7/62;C12P7/64 |
主分类号 |
C12P7/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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