| 摘要 |
<p>Biochip, which is based on silicon, and is manufactured in photolithography and etching technology, comprises many individual wells, which are applied on top silicon-, silicon dioxide- or metal oxide-layer of silicon based chips, and are used as nano-reaction chambers (reaction chambers) for biochemical reactions, in which biological or biochemical reactions take place in aqueous solution, preferably antigen-antibody reactions for analysis and/or detection of enzyme reactions by antigen-antibody reaction. Each microscopic reaction chamber is obtained by conductive silicon structures. Biochip, which is based on silicon, and is manufactured in photolithography and etching technology, comprises many individual wells, which are applied on top silicon-, silicon dioxide- or metal oxide-layer of silicon based chips, and are used as nano-reaction chambers (reaction chambers) for biochemical reactions, in which biological or biochemical reactions take place in aqueous solution, preferably antigen-antibody reactions for analysis and/or detection of enzyme reactions by an antigen-antibody reaction. Each microscopic reaction chamber is obtained by conductive silicon structures, which are used as electrodes for a direct current- or alternating current-based conductivity measurement of the liquid in the reaction chambers. Independent claims are also included for: (1) an apparatus comprising the biochip, which includes small spheres, preferably beads, which are loaded with antigen, antibody or antigen/antibody complex or involved in the analysis, are applied to the chip, and a bead, which of the same size as that of these beads, fits into one of the microscopically small reaction chambers on the chip; and (2) a device for carrying out the antigen-antibody-based analysis in the reaction chambers, preferably for carrying out enzyme-linked immunosorbent assay (ELISA) using the bead as a solid phase, where ELISA assay is monitored and detected by measuring the conductivity. The bead is introduced into each reaction chamber, which is loaded with a first antibody or antigen. A second antibody (detection antibody) bound to the enzyme, which converts a substrate (alkaline phosphatase, e.g. the split ring of p-nitrophenyl phosphate), is added after at least one washing step and binding of the analyte to be measured to the first coating antibody placed on the magnetic bead. The detection of the test reaction is carried out by measuring the conductivity change during the substrate conversion by the enzyme. The binding of analyte to the bead loaded with coating antibody and binding of the detection antibody can take place outside the reaction chamber. The antigen loaded beads are used to detect antibodies in the test material.</p> |
