| 摘要 |
medicine. SUBSTANCE: method involves separating cells of various organ tissues from abortion fetus of 17- 21 weeks intrauterine development. Suspensions are produced. The suspensions, having increased contents of regional trunk cells, are selected. Separate identified regional trunk cell cultures, regional blast cells possessing high migration activity, differentiated cells, special cells like pancreas beta-cells and biologically active substances containing in the fetal tissues are collected. The number of transplant cells varies from 25.104 to 10.107. Viability of cells composing the transplant is not less than 80-90%, II class histological compatibility antigen expression is not greater than 8-15%, spontaneous activity of Ca2+/Mg2+-dependent endonuclease is not less than 0.1 conditional units, induced activity of Ca2+/Mg2+-dependent endonuclease is from 0.3 to 9 conditional units, CD95 marker representation rate is not greater than 5%. The cellular transplant optionally has additions like growth factors and/or migration factors, and/or specific proteins, and/or antioxidation agents and so on. EFFECT: enhanced effectiveness of treatment; high safety level of cellular transplants produced from fetal tissues. 10 cl |
