| 摘要 |
A Bacillus barbaricus strain SCSIO 02429(CCTCC NO: M2010213) derived from sea is provided. Also provided is a method for preparing small squid peptide, which comprises adding Bacillus barbaricus strain SCSIO 02429 to fermentation medium inducing enzyme production to ferment and obtain crude enzyme solution used to hydrolyze, then crushing squid livers into pulp, adding the crude enzyme solution into the pulp to enzymatically digest it and obtain crude protein hydrolysate the glycosyl side chains of which are destroyed, adding bromelain to enzymatically digest, keeping the hydrolystae obtained on standing to separate crude fat from water layer, removing fat layer and drying the remains to obtain small squid peptides. The yield rate of small squid peptides by the method of the present application is 40-46%, and the yield rate of amino acids is 16-22%. The small squid peptides can be used to decrease mortality of young maricultural animals and improve growth rate. The small squid peptides also can be used to replace the traditional protein sources in feed such as fish meal, and can be used as functional protein sources and additives in maricultural formulated feed. |
