| 摘要 |
A fluorescence spectrum recording unit, a fluorescence image acquiring unit, and a fluorescent dye density-calculating unit are comprised, wherein, when a1 (»1) to am (»n) denote the coefficients for the fluorescent dyes 1 to m at the standard density at the wavelengths »1 to »n which are recorded on the recording unit respectively, I all (»1) to I all (»n) denote the intensities of the fluorescence image acquired by the acquiring unit at the wavelengths »1 to »n respectively, and D1 to Dm denote the densities of the fluorescent dyes 1 to m respectively, the calculating unit calculates the densities D1 to Dm in each of all the pixels and in all of the pixels with the following equation, and, in the case where there exists a pixel in which one of the calculation values of the densities D1 to Dm is smaller than 0, a set value which is larger than the calculation value smaller than 0 is substituted for the density the calculation value of which is smaller than 0 in the equation, and the densities of the other fluorescent dyes are recalculated, relative to the pixel: |
