发明名称 |
COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS |
摘要 |
Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant RNAi construct that provides for the inhibition of expression of α1,3-fucosyltransferase (FucT) and &bgr;1,2-xylosyltransferase (XylT) in a plant. Use of these RNAi constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a “humanized” N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided. |
申请公布号 |
HK1118309(A1) |
申请公布日期 |
2013.01.18 |
申请号 |
HK20080111335 |
申请日期 |
2008.10.14 |
申请人 |
SYNTHON BIOPHARMACEUTICALS B.V. |
发明人 |
DICKEY, LYNN, F.;COX, KEVIN, M.;PEELE, CHARLES, G. |
分类号 |
A01H;C07K;C12N;C12P |
主分类号 |
A01H |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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