摘要 |
<P>PROBLEM TO BE SOLVED: To provide parthenogenetically activated human oocytes and human embryonic stem cells produced using the same. <P>SOLUTION: Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O<SB POS="POST">2</SB>tension, including manipulation of Ca<SP POS="POST">2+</SP>under high O<SB POS="POST">2</SB>tension and contacting oocytes with serine threonine kinase inhibitors under low O<SB POS="POST">2</SB>tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high O<SB POS="POST">2</SB>tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing ICM/stem cells. The stem cells produced by the disclosed methods are also described. <P>COPYRIGHT: (C)2013,JPO&INPIT |