FACS-BASED METHOD FOR OBTAINING AN ANTIBODY SEQUENCE
摘要
In certain embodiments, the method may comprises: a) contacting a population of permeabilized, cross-linked antibody-producing cells with a labeled antigen to produce a labeled sample in which cells that produce an antibody that specifically binds to said antigen are intracellularly labeled; b) using FACS to isolate cells that are intracellularly labeled, thereby producing labeled cells; c) uncrosslinking said labeled cells to produce uncrosslinked cells; and d) amplifying heavy and light chain-encoding nucleic acid from individual uncrosslinked cells, thereby obtaining nucleic acid that encodes the variable domain of antibody that specifically binds to said antigen.
申请公布号
WO2013009767(A2)
申请公布日期
2013.01.17
申请号
WO2012US46098
申请日期
2012.07.10
申请人
EPITOMICS, INC.;KE, YAOHUANG;YU, GUO-LIANG;VROOM, KARIN