发明名称 |
HCV replicons containing NS5B from genotype 2B |
摘要 |
The present invention features methods for enhancing the ability of a genotype 2b NS5B sequence to function in a replicon, for producing replicons containing a functional genotype 2b NS5B, and for using replicons to measure the ability of a compound to affect HCV replication that is sustained with the genotype 2b polymerase. Also featured is a genotype 1b NS4B adaptive mutation. The ability to produce replicons containing a functional genotype 2b NS5B is illustrated by the production of chimeric replicons based on HCV genotype 1b where substantially all the NS5B sequence is replaced with a genotype 2b NS5B.
|
申请公布号 |
US8354518(B2) |
申请公布日期 |
2013.01.15 |
申请号 |
US20040577893 |
申请日期 |
2004.11.03 |
申请人 |
MERCK SHARP & DOHME CORP.;ISTITUTO DI RICHERCHE DI BIOLOGIA MOLECOLARE P. ANGELETTI S.P.A.;LUDMERER STEVEN W.;GRAHAM DONALD J.;LAFEMINA ROBERT L.;FLORES OSVALDO A.;PIZZUTI MAURA;TRABONI CINZIA |
发明人 |
LUDMERER STEVEN W.;GRAHAM DONALD J.;LAFEMINA ROBERT L.;FLORES OSVALDO A.;PIZZUTI MAURA;TRABONI CINZIA |
分类号 |
C07H21/04;C12N;C12N7/00;C12N15/00;C12P21/00;C12Q1/70 |
主分类号 |
C07H21/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|