发明名称 |
Polypeptide producing cells |
摘要 |
The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
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申请公布号 |
US8354516(B2) |
申请公布日期 |
2013.01.15 |
申请号 |
US20070299918 |
申请日期 |
2007.05.15 |
申请人 |
HOFFMANN-LA ROCHE INC.;ENDL JOSEF;KOPETZKI ERHARD;PLOETTNER OLIVER;SCHWARZ URSULA;TIEFENTHALER GEORG |
发明人 |
ENDL JOSEF;KOPETZKI ERHARD;PLOETTNER OLIVER;SCHWARZ URSULA;TIEFENTHALER GEORG |
分类号 |
C07H21/04;C12N15/00 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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