| 摘要 |
<P>PROBLEM TO BE SOLVED: To obtain a recombinant nucleic acid molecule including a selective marker useful for the transformation of a microorganism of the Order Thraustochytriales, and to develop a transformation method using the recombinant nucleic acid molecule. <P>SOLUTION: There are provided: nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each derived from a Thraustochytriales microorganism; recombinant vectors useful for transformation of Thraustochytriales microorganisms; and the method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms. <P>COPYRIGHT: (C)2013,JPO&INPIT |
