ZINC FINGER NUCLEASE TARGETING THE CMAH GENE, AND A USE THEREFOR

摘要

The present invention relates to: a method for making pig cells from which the swine CMAH (swine cytidine monophospho-N-acetylneuraminic acid hydroxylase) gene has been knocked out; a method for making a pig from which the swine CMAH gene has been knocked out; a method for making a pig-derived artificial organ or artificial tissue from which the swine CMAH gene has been knocked out; a zinc finger nuclease targeting the CMAH gene; a polynucleotide coding for the zinc finger nuclease; a vector comprising the polynucleotide; a method for making cells from which the CMAH gene has been knocked out by using the zinc finger nuclease; and an egg of a nonhuman animal from which the CMAH gene has been knocked out, the nonhuman animal and a kit used to make an artificial organ derived from the nonhuman animal. The present invention makes it possible to obtain a large volume of transgenic cells for creating pigs for xenogeneic organ transplantation from which the CMAH gene is eliminated, by using a zinc finger nuclease for CMAH genes.