| 发明名称 |
METHOD FOR PREPARING SUBSTANCE L-LYSINE-ALPHA-OXYDASE |
| 摘要 |
FIELD: medicine.SUBSTANCE: what is presented is a method for preparing the substance L-lysine-alpha-oxydase (LO) with the use of the Trichoderma cf. aureoviride Rifai BKMF-4268D producer strain. It is followed by fermentation on a medium containing nitrogen, phosphate sources and wheat bran. It is followed by recovery and purification of the enzyme in the following sequence of the procedures: culture fluid dialysis, adsorbent treatment, impurity sedimentation in 20%-saturated ammonium, hydrophobic and ion-exchange chromatography. It is followed by preparing L-lysine-alpha-oxydase of molecular weight 115-116 kDa, isoelectric point 4.25, optimum pH - 7.4.EFFECT: higher yield of LO.5 tbl, 5 ex |
| 申请公布号 |
RU2471866(C1) |
申请公布日期 |
2013.01.10 |
| 申请号 |
RU20110142641 |
申请日期 |
2011.10.24 |
| 申请人 |
FEDERAL'NOE GOSUDARSTVENNOE BJUDZHETNOE OBRAZOVATEL'NOE UCHREZHDENIE VYSSHEGO PROFESSIONAL'NOGO OBRAZOVANIJA "ROSSIJSKIJ UNIVERSITET DRUZHBY NARODOV"(RUDN);UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT BIOKHIMII I FIZIOLOGII MIKROORGANIZMOV IM. G.K. SKRJABINA RAN (IBFM RAN);FEDERAL'NOE GOSUDARSTVENNOE UNITARNOE PREDPRIJATIE "GOSUDARSTVENNYJ NAUCHNYJ TSENTR "NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUT ORGANICHESKIKH POLUPRODUKTOV I KRASITELEJ" (FGUP "GNTS "NIOPIK") |
发明人 |
BEREZOV TEMIRBOLAT TEMBOLATOVICH;LUKASHEVA ELENA VASIL'EVNA;TRESHCHALINA ELENA MIKHAJLOVNA;ARINBASAROVA ANNA JUR'EVNA;MEDENTSEV ALEKSANDR GRIGOR'EVICH;KISELEVSKIJ MIKHAIL VALENTINOVICH;POKROVSKIJ VADIM SERGEEVICH;BORONIN ALEKSANDR MIKHAJLOVICH;BARYSHNIKOV ANATOLIJ SERGEEVICH |
| 分类号 |
C12N9/02;C12N9/00;C12N9/04;C12R1/885 |
主分类号 |
C12N9/02 |
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