| 摘要 |
A fluorescence-spectrum-recording unit, a fluorescence-image-acquiring unit, and a fluorescent-dye-density-calculating unit are comprised, the calculating unit calculates the densities D1 to Dm of fluorescent dyes 1 to m in each of all the pixels and in all of the pixels with the following equation, and, when there exists a pixel in which one of the calculation values of these densities is smaller than 0, a set value larger than the calculation value smaller than 0 is substituted for the density the calculation value of which is smaller than 0 in the equation, and the densities of the other fluorescent dyes are recalculated, relative to the pixel: ( D   1 ⋮ Dm ) = ( a   1  ( λ   1 ) … am  ( λ   1 ) ⋮ ⋮ ⋮ a   1  ( λ   n ) … am  ( λ   n ) ) - 1  ( I all  ( λ   1 ) ⋮ I all  ( λ   n ) ) where a1 (λ1) to am (λm) denote the coefficients for the fluorescent dyes at the standard density at the wavelengths λ1 to λn, and Iall (λ1) to λall (λn) denote the intensities of the fluorescence image at the wavelengths λ1 to λn.
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