发明名称 |
METHOD FOR PURIFYING POLYPEPTIDE SOLUTIONS |
摘要 |
Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.
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申请公布号 |
US2013005948(A1) |
申请公布日期 |
2013.01.03 |
申请号 |
US201113582606 |
申请日期 |
2011.03.09 |
申请人 |
BINDER VINZENZ;HAKEMEYER CHRISTIAN;SCHWARZ FELIZITAS |
发明人 |
BINDER VINZENZ;HAKEMEYER CHRISTIAN;SCHWARZ FELIZITAS |
分类号 |
C07K1/14 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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