发明名称 |
SMOOTH MUSCLE-LIKE CELLS (SMLCs) DERIVED FROM HUMAN PLURIPOTENT STEM CELLS |
摘要 |
This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into smooth muscle-like cells (SMLCs) in vitro, comprising a) plating a single-cell suspension of PSCs that are smaller than 50 μm at a seeding concentration of about 5×104 cells/cm2-about 1×105 cells/cm2 onto a suitable surface, and culturing the cells under conditions which prevent the PSCs from aggregating and induce differentiation of the PSCs into vasculogenic progenitor cells; b) harvesting the cultured cells of step a) and separating them into a single cell suspension of cells that are smaller than 50 μm; and c) plating the single cell suspension of step b) at a seeding concentration of about 1×104 cells/cm2-about 5×104 cells/cm2 on a suitable surface, and culturing the cells in a differentiation medium that is supplemented with platelet-derived growth factor BB (PDGF-BB) and transforming growth factor-beta 1 (TGF &bgr;1), for a sufficient period of time to allow the vasculogenic progenitor cells to mature into SMLCs. In one embodiment of the invention, the cells from step c) are further subjected to a shear force of at least 1 dyne/cm2 for a time period sufficient to enhance differentiation, maturation and/or functionality of the cells. |
申请公布号 |
EP2539437(A2) |
申请公布日期 |
2013.01.02 |
申请号 |
EP20110748168 |
申请日期 |
2011.02.25 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
GERECHT, SHARON;HANJAYA-PUTRA, DONNY;VO, ELAINE, TUONG, VI;WANJARE, MAUREEN |
分类号 |
C12N5/071;C07K14/475;C12N5/02;C12N5/0735 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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地址 |
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