发明名称 |
Metabolically engineered cells for the production of pinosylvin |
摘要 |
A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.
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申请公布号 |
US8343739(B2) |
申请公布日期 |
2013.01.01 |
申请号 |
US20070374659 |
申请日期 |
2007.07.19 |
申请人 |
FLUXOME SCIENCES A/S;KATZ MICHAEL;FOERSTER JOCHEN;DAVID HELGA;SCHMIDT HANS PETER;SENDELIUS MALIN;BJOERN SARA PETERSON;DURHUUS THOMAS THOMASEN |
发明人 |
KATZ MICHAEL;FOERSTER JOCHEN;DAVID HELGA;SCHMIDT HANS PETER;SENDELIUS MALIN;BJOERN SARA PETERSON;DURHUUS THOMAS THOMASEN |
分类号 |
C12P7/22 |
主分类号 |
C12P7/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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