发明名称 |
Regio- and enantioselective alkane hydroxylation with modified cytochrome P450 |
摘要 |
Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.
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申请公布号 |
US8343744(B2) |
申请公布日期 |
2013.01.01 |
申请号 |
US20110983841 |
申请日期 |
2011.01.03 |
申请人 |
THE CALIFORNIA INSTITUTE OF TECHNOLOGY;ARNOLD FRANCES H;PETERS MATTHEW W;MEINHOLD PETER |
发明人 |
ARNOLD FRANCES H;PETERS MATTHEW W;MEINHOLD PETER |
分类号 |
C12N9/02;C07H21/04;C12N;C12N15/00;C12P7/00;C12P21/04;C12P21/06 |
主分类号 |
C12N9/02 |
代理机构 |
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地址 |
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