A NOVEL PLASMID FROM LACTIC ACID BACTERIA AND LAB-E.COLI SHUTTLE VECTOR COMPRISING THE SAME

摘要

PURPOSE: A shuttle vector comprising plasmid from lactic acid bacteria is provided to stably express a gene or protein and to be able to reproduce over 100 generations due to a stable method of theta-typed reproduction. CONSTITUTION: Plasmid pMBLT00 comprises the sequence number marked sequence number 1(SEQ ID NO:1). Plasmid pMBLT00 is separated Leuconostoc mesenteroides. A shuffle vector capable of reproduction both in LAB-E-coli and lactic acid bacteria comprises sequence number marked the sequence number 1 and produced by using plasmid pMBLT00 and transformation vector from LAB-E-coli. The transformation vector from LAB-E-coli is pUC19. The shuffle vector has a method of theta-typed reproduction. A transformant transformed from the shuffle vector is LAB-E-coli or lactic acid bacteria. Lactic acid bacteria are Lactococcus, Leuconostoc or Pediococcus. A method of reproduction comprises the following steps. The shuffle vector capable of reproduction both in LAB-E-coli and lactic acid bacteria is made by using plasmid pMBLT00 with the sequence number marked the sequence number 1 and transformation vector from LAB-E-coli(a). The recombinant vector is made combining the shuffle vector comprising a target gene which codes target proteins(b). The recombinant vector is injected into microorganism.