SUBSTRATE AGAINST PROTEASE ISOLATED FROM MICROORGANISM, WITH WIDE RANGE- SUBSTRATE SPECIFICITY, AND USE THEREFOR

摘要

PURPOSE: A fluorescent substrate for protease with broad substrate specificity and a diagnostic kit containing the same are provided to accurately measure protease activity and to identify bacteria presence. CONSTITUTION: A flurescent substrate is specific to secretory protease and has chemical formula 1(MCA-Xn-A-Xm-DNP). The bacteria are vibrio vulnificus including Vibrio vulnificus, Vibrio mimicus, Vibrio fluvialis, Vibrio furnissii, Vibrio hollisae, Vibrio alginolyticus, Vibrio cincinnatiensis, or Vibrio metschnikovii. The Vibrio vulnificus is Vibrio vulnificus ATCC29307. The protease is metalloprotease. A diagnostic kit contains the fluorescent substrate. The kit is used for measuring the presence and content of the protease by MCP luminescence.