METHOD FOR PREPARING NESIRITIDE

摘要

Provided by the present invention is a method for preparing nesiritide, comprising successively linking corresponding Fmoc-protected amino acid in the sequence of nesiritide on Fmoc-His (trt)-carrier resin by solid phase coupling synthesis process, wherein only one or two solid phase coupling reaction(s) is/are used in the linking of Ser(tBu)-Ser(tBu)-Ser(tBu)-Ser(tBu) fragment, and the Fmoc-protected amino acid uesed is at least one of Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-Ser(tBu)-Ser(tBu)-OH, Fmoc-Ser(tBu)-Ser(tBu)-Ser(tBu)- Ser(tBu)-OH, Fmoc-Ser(tBu)-Ser(?Me,Mepro)-OH and Fmoc-Ser(tBu)-Ser(tBu)-OH.