RECOMBINANT EXPRESSION TECHNIQUE FOR DISULFIDE-BRIDGED, DOUBLE-STRANDED PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a double-stranded polypeptide or protein by recombinant expression in an E. coli host cell.

SOLUTION: The method for producing the polypeptide or protein comprises: a step of modifying a polypeptide or a protein in nucleic acid level; a step of introducing the product modified in nucleic acid level into an E. coli cell; a step of culturing and dissolving the host cell; and a step of isolating the double-stranded polypeptide or protein. The polypeptide or protein is a botulinum neurotoxin, especially botulinum neurotoxin type A (BoNT(A)). The polypeptide or protein exerts its biological activity as a double-stranded polypeptide or protein.

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