PHARMACEUTICAL COMPOSITION FOR TREATING ISCHEMIC DISEASES, CONTAINING CONDITIONED MEDIUM OBTAINED THROUGH THREE-DIMENSIONAL CELL CULTURE AS ACTIVE INGREDIENT

摘要

The present invention provides a pharmaceutical composition for treating ischemic diseases, containing a conditioned medium obtained from a cultured product obtained through three-dimensional culturing of adult stem cells in a medium for cell culture, as an active ingredient. The concentration and content of an angiogenesis promoting factor of the conditioned medium according to the present invention is very high compared with those of a conditioned medium obtained through a two-dimensional culture method, and thus the conditioned medium of the present invention can be useful for the vascular regeneration in ischemic vascular diseases. In addition, the conditioned medium can be clinically applied safely since there are no animal-derived ingredients, a buffer solution and indicator ingredients which have been used in a conventional cell culture medium or conditioned medium, and the conditioned medium can be effectively used in ischemic vascular diseases and the like even in small amounts.