| 摘要 |
Artificial packaging RNA (pRNA) polynucleotides that share secondary structure with dsDNA bacteriophage pRNA (e.g., phi29 pRNA) are formed by non-covalent self-assembly from two RNA pieces to form pRNA monomers that may include heterologous sequences to form chimeric pRNA monomers. The two RNA pieces (P1 and P2) can be made by non-enzymatic chemical synthesis and the resulting monomer includes a break in the single- stranded head loop corresponding to positions 53-58 in a wild-type phi29 pRNA. The synthetic pRNA monomers can be assembled into multimeric polyvalent nanoparticles and may include as heterologous components payloads such as siRNA, ribozymes, aptamers, antisense RNA, detectable labels, therapeutic drugs and the like, as well as targeting moieties, endosome- disrupting agents and other moieties. |
