| 摘要 |
It is an object of the present invention to provide a method for separating and obtaining a basic amino acid hydrochloride from a basic amino acid fermentation broth or an enzyme reaction solution which enzyme reaction has been catalyzed by viable cells of a basic amino acid-producing microbe, each containing sulfate ions, by which method product yields and qualities are almost the same and are secured more easily, as compared with the conventional technique, which method comprises the steps of: (1) adding the chloride of a metal selected from the group consisting of calcium, potassium, magnesium and barium to the basic amino acid fermentation broth or the enzyme reaction solution which contains the sulfate ions to precipitate the sulfate ions as crystals of the resulting metal sulfate, (2) removing the metal sulfate crystals from the basic amino acid solution, (3) cooling the basic amino acid fermentation broth or the enzyme reaction solution which the metal sulfate crystals are removed from while keeping the concentration of the metal sulfate below its saturated concentration to precipitate the basic amino acid as its hydrochloride crystals, and (4) separating the basic amino acid hydrochloride crystals to collect them. |
