METHOD FOR IDENTIFYING TARGET PROTEIN OF ANTIMICROBIAL AGENT BASED ON ANALYSIS OF DRUG-RESISTANT AND TEMPERATURE-SENSITIVE MUTANT STRAIN

摘要

PROBLEM TO BE SOLVED: To provide a method for identifying a target protein of "antimicrobial agent" such as an antibiotic, and a method of identifying a base substitution causing a drug resistance to be obtained.

SOLUTION: The method for identifying the target protein of the antimicrobial agent includes: isolating a temperature-sensitive mutant strain from among drug-resistant mutant strains against the agent; identifying the protein into which an amino acid mutation in the temperature-sensitive mutant is introduced; and identifying a protein before the amino acid mutation is introduced, as the target protein of the antimicrobial agent.

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