Novel 7alpha-hydroxysteroid dehydrogenase knockout mutants and use thereof

摘要

The invention relates to novel microbial 7α-hydroxysteroid dehydrogenase (7α-HSDH) knockout mutants and to the use thereof for producing other HSDHs having various functionalities, such as 3α-, 7ß- or 12α-HSDH, and to the use of thus-produced HSDH enzymes in enzymatic reactions of cholic acid compounds, and in particular for producing ursodeoxycholic acid (UDCS). The invention relates in particular to novel methods for synthesizing UDCS.