A method for releasing the content of the periplasmic space of bacterial celis is provided, which comprises incubating the bacterial cells in a solution containing styrene maleic acid copolymer (SMA). Also provided is a method of preparing a substantially pure sample of recombinant polypeptide. The methods find application in the recovery of materials, such as proteins, from bacterial cells.
申请公布号
WO2012153089(A8)
申请公布日期
2012.12.20
申请号
WO2012GB00423
申请日期
2012.05.09
申请人
THE UNIVERSITY OF BIRMINGHAM;DAFFORN, TIMOTHY;TYRYNIS-THOMAS, OWEN ROBERT