发明名称 |
METHOD FOR CONTROLLING DIFFERENTIATION OF STEM CELL USING HUMAN H3K79 METHYLTRANSFERASE DOT1L |
摘要 |
PURPOSE: A method for controlling stem cell differentiation using hDot1L which controls pluripotency-related gene expression at a genome level is provided to control the differentiation. CONSTITUTION: A method for changing differentiation ability of stem cells comprises a step of controlling intracellular level of hDot1L(human Dot1 like). A method for suppressing stem cell differentiation comprises a step of increasing intracellular level of hDot1L. A method for promoting stem cell differentiation comprises a step of reducing intracellular level of hDot1L(human Dot1 like). The stem cells include human stem cell H9, H1, CHA3, and CHA4. A composition for promoting stem cell differentiation contains hDot1L expression inhibitor as an active ingredient. A composition for suppressing stem cell differentiation contains a promoter, and a vector containing a nucleotide encoding hDot1L, which is operatively linked to the promoter.
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申请公布号 |
KR20120136234(A) |
申请公布日期 |
2012.12.18 |
申请号 |
KR20110055322 |
申请日期 |
2011.06.08 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
KIM, SEUNG KYOON;LEE, DAE YOUP |
分类号 |
C12N5/0735;C12N9/10;C12N15/11;C12N15/54 |
主分类号 |
C12N5/0735 |
代理机构 |
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主权项 |
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地址 |
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